Anti-Sodium-Iodine Symporter (NIS) C-term (C15-1316-648)

General description

Sodium-Iodide Sympoter (Uniprot: Q63008; also known as NIS, Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5) is encoded by the Scl5a5 (also known as Nis) gene (Gene ID: 114613) in rat. NIS is a multi-pass membrane protein that is involved in the transport of iodide in thyroid gland. It is highly expressed in thyroid epithelial cells and lower levels of expression are also reported in mammary gland, salivary gland, stomach and colon. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. Nis gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of Nis gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.

Specificity

This polyclonal antibody detects sodium/iodide co-transporter in rat thyroid gland. It targets an epitope within 16 amino acids from the C-terminus region.

Immunogen

Epitope: C-terminus

KLH-conjugated liner peptide corresponding to 16 amino acids from the C-termnus region of rat Sodium/iodide cotransporter.

Application

Anti-Sodium-Iodine Symporter (NIS) C-term, Cat. No. ABC1453, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A 1:1000-1:40K dilution from a representative lot detected Sodium-Iodine Symporter (NIS) C-term in FRTL-5, thyroid cells (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University).

Immunocytochemistry Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Immunocytochemistry applications (Levy, O., et. al. (1997). Proc Natl Acad Sci USA. 94(11):5568-73; De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Immunohistochemistry Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in rat thyroid, mammary gland from lactating rat (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).

Western Blotting Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Western Blotting applications (Levy, O., et. al. (1997). Proc Natl Acad Sci USA. 94(11):5568-73; Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8; Levy, O., et. al. (1998). J Biol Chem. 273(35):22657-63; De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

Immunoprecipitation Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Immunoprecipitation applications (De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Levy, O., et. al. (1998). J Biol Chem. 273(35):22657-63).

Quality

Evaluated by Western Blotting in rat thyroid tissue lysate.

Western Blotting Analysis: 1.5 µg/mL of this antibody detected Sodium-Iodine Symporter (NIS) C-term in 10 µg of rat thyroid tissue lysate.

Target description

~80 kDa observed; 65.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 2 M Tris, 0.2 M glycine buffer (pH 7.4) without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_443215. UniProt accession no.: Q63008. shipped in: ambient. target post-translational modification: unmodified. Gene Information: rat ... Slc5A5(114613). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.