Anti-SOX9 Antibody (C15-1317-205)

General description

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Immunogen

Linear peptide corresponding to the C-terminus of human SOX9.

Application

Anti-SOX9 Antibody is a highly specific rabbit polyclonal antibody, that targets SOX9 & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SOX9 in K14 mouse backskin tissue (Nowak, J. A., et al. (2008). Cell Stem Cell. 3(1):33-43.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected SOX9 in mouse Label Retaining cells, and developing epithelial skin and hair follicle cells (Nowak, J. A., et al. (2008). Cell Stem Cell. 3(1):33-43.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SOX9 in 10 µg of NIH/3T3 cell lysate.

Target description

~63 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. species reactivity (predicted by homology): rat (based on 100% sequence homology), human (based on 100% sequence homology). technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_000337. UniProt accession no.: P48436. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SOX9(6662). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.