ANTI-SPINOPHILIN/NEURABIN-II ANTIBODY

General description

Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.

Specificity

Other species not known.

Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.

Immunogen

GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.

Application

Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on a rat brain microsomal preparation.

Western Blot Analysis:
0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).

Target description

120 kDa

Physical form

Format: Purified

Protein G Purified

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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