ANTI-SPT5 CLONE 6F1

General description

Transcription elongation factor SPT5 (UniProt: O00267; also known as hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DSIF p160, DRB sensitivity-inducing factor large subunit, DSIF large subunit, Tat-co-transactivator 1 protein, Tat-CT1 protein) is encoded by the SUPT5H (also known as SPT5, SPT5H) gene (Gene ID: 6829) in human. SPT5 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF is a heterodimer of SPT4 and SPT5 that positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing can facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II, which binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3′ terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. SPT5 can be methylated by PRMT1/HRMT1L2 and PRMT5/SKB1 and this methylation negatively regulates interaction with the positive transcription elongation factor (P-TEFb) and RNA polymerase II.

Specificity

Clone 6F1 specifically detects SPT5 in HEK293T and Jurkat cells. It targets an epitope of 18 amino acids within the C-terminal half.

Immunogen

Ovallbumin-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Transcription elongation factor SPT5.

Application

Anti-SPT5, clone 6F1, Cat. No. MABE1803, is a highly specific rat monoclonal antibody that targets Transcription elongation factor SPT5 and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SPT5 in 10 µg of HEK293T cell lysate.

Chromatin Immunoprecipitation Analysis: A representative lot detected SPT5 in Chromatin Immunoprecipitation applications (Kelso, T.W., et. al. (2014). Mol Cell Biol. 34(19):3675-88).

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SPT5 in 10 µg of Jurkat cell lysate.

Target description

~121 kDa observed; 121 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.