Anti-SPTLC1 Antibody (C15-1317-756)

General description

Serine palmitoyltransferase 1 (EC 2.3.1.50; UniProt O15269; also known as LCB 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, SPT 1, SPT1) is encoded by the SPTLC1 (also known as HSAN1, HSAN1A, LCB1) gene (Gene ID 10558) in human. Serine palmitoyltransferase (SPT) catalyzes pyridoxal 5-phosphate-/PLP-dependent condensation of L-serine with palmitoyl-CoA to generate 3-ketodihydrosphingosine, the rate-limiting step for the de novo synthesis of sphingolipids. SPT belongs to the POAS (PLP-dependent α-oxoamine synthase) family of enzymes, members of this family also include 5-amino-levulinic acid synthase, KBL (2-amino-3 ketobutyrate ligase) and AONS (8-amino-7-oxononanoate synthase). Unlike the other known POAS family members that all exist as soluble homodimers, SPT is bound to the outer membrane of the endoplasmic reticulum (ER) as a multisubunit complex composed of three distinct subunits, SPTLC1, SPTLC2 and SPTLC3. SPTLC1 is a 473-amino acid sigle-transmembrane (a.a. 16-36) protein with a short N-terminal lumenal domain (a.a. 1-15) and a large C-terminal cytoplasmic domain (a.a. 37-473). Alternative splicing generates an isoform with a truncated cytoplasmic domain (D143 switched to E143, a.a. 144-473 truncated).

Specificity

Expected to react with both spliced isoforms of human Serine palmitoyltransferase 1/SPTLC1 reported by UniProt (O15269).

Immunogen

Epitope: Cytoplasmic domain.

Two linear peptides corresponding to sequences from the N- and a C-terminal regions of human SPTLC1 cytoplasmic domain.

Application

Anti-SPTLC1 Antibody is an antibody against SPTLC1 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Western Blotting Analysis: The purified polyclonal antibody was used to detect the presence of SPTLC1 in SPTLC2 and SPTLC3 immunoprecipiates from human placenta tissue homogenate (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164).
Western Blotting Analysis: The purified polyclonal antibody was used to detect SPTLC1 in human cell lysates (HEK293, HepG2, JAR, JEG3) and human placenta tissue homogenate (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SPTLC1 in 10 µg of HepG2 cell lysate.

Target description

~51 kDa observed. 52.74 kDa (isoform 1) and 16.07 kDa (isoform 2) calculated.

Physical form

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_006406. UniProt accession no.: O15269. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... SPTLC1(10558). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.