General description
Purified mouse monoclonal antibody. Recognizes the SQSTM1 protein.
Recognizes the SQSTM1 protein HeLa cells.
This Anti-SQSTM1 Mouse mAb (2C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of SQSTM1.
Immunogen
Full-length, recombinant human SQSTM1 (aa 1-441), expressed as a GST protein
NA
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Lee, H,K., et al. 2010. Immunity.32, 227.
Bjorkoy, G., et al. 2009. Methods Enzymol.452, 181.
Ferguson, C,J., Lenk, G,M., and Meisler, M,H., Hum. Mol. Genet.18 4868.
Gal, J., et al. 2009. J. Neurochem.111 1062.
Mardakheh, F,K., et al. 2009.J. Cell Biol.187, 265.
Jung, H,S., et al. 2008. Cell Metab.8, 318.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51111756
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- DR1057-100UG