Anti-Stabilin-1 Antibody, CT (C15-1316-418)

General description

Stabilin-1 and Stabilin-2 constitute a novel family of fasciclin domain-containing hyaluronan receptor homologs that may play a role in cell-cell and cell-extracellular matrix interactions in vascular function and inflammatory processes. Stabilin-1 is a transmembrane protein, expressed in endothelial cells within the sinusoidal regions of liver, spleen, and lymph tissues as well as macrophages. Stabilin-2 is absent from the latter (Politz, 2002).

Specificity

Other notable species homologies include mouse (87%) and rat (83%).

The antibody recognizes the C-terminus of Stabilin-1 within the cytoplasmic region of a cell.

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human Stabilin-1.

Application

Anti-Stabilin-1 Antibody, C-terminus is an antibody against Stabilin-1 for use in WB, IH(P) & IF.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Stabilin-1 in human placental tertiary villi.
Immunofluorescence Analysis: A previous lot of Anti-Stabilin-1, C-terminus was shown to positively stain using immunofluorescence techniques (Kzhyshkowska, 2008).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western blot in HUVEC lysate.
Western blot Analysis: 1:1,000 dilution of this antibody detected Stabilin-1 on 10 µg of HUVEC lysate.

Target description

~ 275 kDa

Physical form

Format: Unpurified

Unpurified

Unpurified rabbit polyclonal in serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt if stored in undiluted aliquots.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HUVEC lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: unpurified. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. UniProt accession no.: Q9NY15. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... STAB1(23166). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.