ANTI-STARGAZIN ANTIBODY (C005B-240422)

Specificity

Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum.

Immunogen

GST fusion protein from rat stargazin, cytoplasmic domain.

Application

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin.

Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

ImmunoAffinity Purified

Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4.

Storage and Stability

Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_006078.2. UniProt accession no.: Q9Y698. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Cacng2(12300)
rat ... Cacng2(84347). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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