ANTI-STK3 CLONE 1H12 ZOOMAB RABBIT MONOCLONAL (C15-1669-548)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H12 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Serine/threonine-protein kinase 3 (STK3/MST-2). It targets an epitope with 22 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal region of human Serine/threonine-protein kinase 3 (STK3/MST-2).

Application

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected STK3 in L6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STK3 in Raw264.7 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound STK3 with a KD of 7.7 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected STK3 in human placenta and human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected STK3 in Raw264.7 cells..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Serine/threonine-protein kinase 3 (UniProt: Q13188; also known as EC:2.7.11.1, Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1) is encoded by the STK3 (also known as KRS1, MST2) gene (Gene ID: 6788) in human. STK3 is a homodimeric protein that serves as a stress-activated, pro-apoptotic kinase. It is expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung, and brain tissues. Full activation of STK3 requires its homodimerization and autophosphorylation at threonine 180. Caspase-3 dependent cleavage of STK3 produces a 36 kDa (MST-2/N) and a 20 kDa ST2/C form. Following its cleavage by caspase-3 between residues 322-323, the 36 kDa (MST-2/N) form enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Its phosphorylation at threonine 117 causes inhibition of its cleavage, kinase activity, nuclear translocation, and autophosphorylation at threonine 180. STK3 is a key component of the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting cell proliferation and promoting apoptosis. The activated STK3 kinase negatively regulate the yes-associated protein 1 (YAP1) and WW Domain-containing transcription regulator protein 1 (TAZ), which trigger the expression of proliferation genes and inhibit apoptosis to promote carcinogenesis. However, it is also reported to promote the migration of CD8+ cells by activating NF-kB signaling and suppress ovarian tumor growth. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, B., et al. (2021). Mol. Cancer. 20; Article 147; Wang, X., et al. (2020). J. Immunol. Res. 2020; 7263602; Cooper, WN., et al. (2009). Oncogene. 28(33); 2988-2998).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.