Anti-Substance P Receptor Antibody, pain (C15-1316-175)

General description

Substance P is a tachykinin family neuropeptide that functions as a neurotransmitter and neuromodulator. In the CNS, Substance P is involved in the regulation of mood, anxiety, sleep, saiety, reinforcement, respiratory rhythm and pain processing. In nociception, the neuropeptide is released at local spinal cord synapses. Recent research now shows substance P is potentially involved in endothelial cell differentiation as well as diverse roles in neuroinflammatory response. The endogenous receptor for Substance P is neurokinin 1 receptor (NK1 receptor), which belongs to the tachykinin receptor sub-family of GPCRs.

Specificity

Specific for the Substance P Receptor (NK-1).

Immunogen

Epitope: C terminus

Synthetic peptide that corresponds to a 23 amino acid sequence (385-407) of the COOH terminus of the rat Substance P Receptor (NK-1).

Application

Anti-Substance P Receptor Antibody, pain detects level of Substance P Receptor & has been published & validated for use in IF, IH & WB.

Immunohistochemistry:
A previous lot was used at 1:100-1:1,000 dilution.

Western Blot Analysis:
A previous lot was used at 1:500 dilution.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuroinflammation & Pain

Quality

Evaluated by western blot on Mouse brain membrane lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected NK-1R on 10 μg of Mouse brain membrane lysates.

Target description

35 kDa

Linkage

Replaces: AB5800

Physical form

Rabbit polyclonal serum containing no preservative.

Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Spinal Cord, brain membrane lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, guinea pig, mouse. manufacturer/tradename: Chemicon^®. . technique(s): immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_003182.1. UniProt accession no.: P20366. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... TAC1(6863). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.