General description
Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
Specificity
This antibody recognizes the large T antigen of SV40.
Immunogen
Epitope: Large T antigen of SV40
Purified large T-antigen of SV40
Application
Anti-SV40 Large T Antigen Antibody, clone PAb416 is an antibody against SV40 Large T Antigen for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected SV40 Large T Antigen in ICC (Sabatier, J., et al. (2005). 58(4):429-431.; Del Valle, L., et al. (2004). J Virol. 78(7):3462-3469.).
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Quality
Evaluated by Western Blot in Cos-1 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected SV40 Large T Antigen in 10 µg of Cos-1 cell lysate.
Target description
~82 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Cos-1 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51201649
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABF121
- Temperature Control Device:
- Yes
