General description
Switch-associated protein 70 (UniProt: Q9UH65; also known as SWAP-70) is encoded by the SWAP70 (also known as KIAA0640, HSPC321) gene (Gene ID: 23075) in human. SWAP-70 is a phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent guanine nucleotide exchange factor (GEF) that, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. SWAP-70 regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. It is expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG, but not IgM. It is also widely expressed in spleen, kidney, lung, and liver. SWAP-70 has an N-terminal putative EF-hand domain, a pleckstrin homology (PH) domain (aa 210-306) that binds phosphatidylinositol 3,4,5-trisphosphate and is responsible for membrane localization, a tri-partite coiled-coil region (aa 316-539), and a C-terminal F-actin-binding site through which it specifically binds non-muscle actin. (Ref.: Chacón-Martínez, CA et al. (2103). J. Biol. Chem. 288(40); 28687-28703).
Specificity
This rabbit polyclonal antibody detects human Switch-associated protein 70. It targets an epitope with in 12 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal region of human Switch-associated protein 70.
Application
Anti-SWAP-70, Cat. No. ABC1688, is a rabbit polyclonal antibody that detects Switch-associated protein 70 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected SWAP-70 in human tonsil and human lung tissue sections.
Quality
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected SWAP-70 in Raji cell lysate.
Target description
~70 kDa observed; 69.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
biological source: rabbit. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_001284643. UniProt accession no.: Q9UH65. target post-translational modification: unmodified. Gene Information: human ... SWAP70(23075). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51342705
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABC1688-25UG