General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
Recognizes the synapsin I protein in rat brain.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.
Immunogen
Bovine
purified, brovine brain synapsin I
Application
ELISA (1:2500-1:10,000)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 5 mM ammonium bicarbonate.
Reconstitution
Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat brain
Other Notes
Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. form: lyophilized. does not contain: preservative. species reactivity: human, rat, mouse, bovine. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. isotype: IgG. shipped in: ambient. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: mouse ... Syn1(20964). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1.- UPC:
- 51473009
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 574777-10UG