Anti-Synuclein alpha Antibody (C15-1316-166)

General description

alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer′s Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).

Specificity

Alpha-synuclein. The immunizing peptide was chosen to reduce cross-reactivity of AB5038 with β-synuclein to a minimum. Immunohistochemical and Western blot analysis of human brain indicates a high level of specificity.

Has not been tested on other species.

Immunogen

Epitope: a.a. 111-131

Human alpha-synuclein residue 111-131 coupled to KLH.

Application

Anti-Synuclein Antibody, α detects level of Synuclein & has been published & validated for use in IC, IH, IH(P) & WB.

Immunohistochemistry:
1:1,000-1:2,000 dilutoin of a previous lot was used on paraffin sections; 1:4,000-1:12,000 dilution of a previous lot was used on frozen sections.

Western blot:
1:1,000-1:2,000. Shows a 14-19kDa band.

Immunocytochemistry:
Previous lot used IC.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by Western Blot on Human Brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Synuclein on 10 µg of human brain lysates.

Target description

~14-19 kDa

Linkage

Replaces: 04-1053

Physical form

Rabbit polyclonal serum lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any residue.

Analysis Note

Control
Human brain sections.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, human. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable. NCBI accession no.: NM_000345.2. UniProt accession no.: P37840. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... SNCA(6622). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.