ANTI-TAF5L CLONE 1B18 ZOOMAB RABBIT MONOCLONAL (C005B-261997)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B18 is ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects TAF5L. It targets an epitope within 18 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human TAF5L.

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TAF5L in Jurkat cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant Human TAF5L.

Enzyme Immunoassay Analysis (ELISA): A representative lot of this antibody detected recombinant Human TAF5L in ELISA application.

Affinity Binding Assay: A representative lot of this antibody bound TAF5L peptide with a KD of 4.4 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L (UniProt: O75529; also known as TAF5L, PCAF-associated factor 65 beta, PAF65-beta) is encoded by the TAF5L (also known as PAF65B) gene (Gene ID: 27097) in human. TAFL5 functions as a component of the PCAF complex that is capable of efficiently acetylating nucleosomal histones. The PCAF complex is composed of a number of TBP-associated factors (TAFS), including TAF5, TAF5L, TAF6, TAF6L, and PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3, and SPT3. TAF5L, in conjunction with TAFL6, acts as a epigenetic regulator that is essential for somatic reprogramming. TAF5l/TAF6L are components of GNAT-HAT complexes. They regulate their target gene expression through H3K9ac deposition and c-Myc recruitment that ultimately trigger the Myc regulatory network for self-renewal of embryonic stem cells. TAF5L contains six WD domains in its C-terminal half. Two isoforms of TAF5L have been described that are produced by alternative splicing. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Seruggia, D., et al. (2019). Mol. Cell. 74(6); 1148-1163; Struhl, K., and Moqtaderi, Z. (1998). Cell. 94(1); 1-4).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.