ANTI-TAU CLONE T49 (NOT HUMAN) (MOUSE MONOCLONAL)

General description

Tau or Microtubule-associated protein tau (MAPT), also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), is a cytosolic protein that promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of axonal polarity in neurons. Tau binds to and is thought to function as a linker protein between axonal microtubules and neural plasma membrane components. There are multiple isoforms, and the short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. PAD is the phosphatase activating domain, and has been demonstrated to be involved in the inhibition of anterograde, kinesin-based fast axonal transport (FAT) by activating axonal protein phosphatase 1 (PP1) and glycogen synthase kinase 3 (GSK3), independent of microtubule binding. Defects in Tau are thought to be the cause of a number of neurodegenerative diseases, including frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND), Pick disease of the brain (PIDB), corticobasal degeneration (CBD), supranuclear palsy type 1 (PSNP1), Alzheimer disease, and Parkinson disease. Clone T49 exhibits immunoreactivity against bovine, rat, and murine, but not human, Tau (UniProt P29172, P19332, P10637, P10636, respectively).

Immunogen

Purified corresponding to bovine Tau.

Application

Anti-Tau Antibody, clone T49 (Not human) is an antibody against Tau for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tau in mouse cerebral cortex, mouse kidney, and mouse small intestine tissue.
Western Blotting Analysis: A representative lot detected Tau in PS19 neurons treated with PBS or transduced with strain A or strain B FL a-syn pffs (Guo, J.L., et al. (2013). Cell. 154:103-117).

Quality

Evaluated by Western Blotting in mouse and human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tau in 10 µg of mouse and human brain tissue lysate.

Target description

~53 kDa observed. This protein has multiple isoforms which range from 45-76 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.