Anti-Tau phospho Serine 404 Antibody (C15-1316-550)

General description

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Serine 404 is phosphorylated by GSK-3beta and cdk5 in vitro and in vivo.

Specificity

Tau phosphoSerine 404. The antibody recognizes Tau pSerine 404 in samples of recombinant human Tau treated with GSK-3beta for 45 minutes. The reactivity of the antibody is blocked with the pSerine 404 peptide but not the non-phosphopeptide or a generic phosphoSerine-containing peptide.

Immunogen

Synthetic peptide of amino acids surrounding the phosphoSerine 404 site of human Tau.

Application

Anti-Tau phospho Serine 404 Antibody is an antibody against Tau phospho Serine 404 for use in WB.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 3% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.


Optimal working dilutions must be determined by the end user.

Packaging

10 blots

Physical form

Affinity purified immunoglobulin. Liquid in Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), pH 7.3, 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_005910.3, NM_016834.2, NM_016835.2, NM_016841.2. UniProt accession no.: P10636. shipped in: dry ice. target post-translational modification: phosphorylation (pSer404). Gene Information: human ... MAPT(4137). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.