Specificity

Taurine. The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.

Immunogen

Taurine-glutaraldehyde

Application

Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.05% - 0.25% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5022 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5022 for your days use by diluting 100X with 1% GSPBT.

This Anti-Taurine (No Gluteraldehyde) Antibody is validated for use in IH for the detection of Taurine (No Gluteraldehyde).

Physical form

IgG fraction in sterile 0.1M phosphate buffer. No preservative

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: diluted serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: vertebrates. manufacturer/tradename: Chemicon^®.. technique(s): immunohistochemistry: suitable. shipped in: wet ice. target post-translational modification: unmodified. Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(C): Not applicable.