ANTI-TBR-2 (C005B-241246)

General description

Eomesodermin homolog (UniProt: O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2) gene (Gene ID: 13813) in murine species. T-box genes are a family of transcription factors have a highly conserved DNA binding domain named the T-domain. TBR-2 functions as a transcriptional activator that plays a crucial role during gastrulation, mesodermal specification, and limb patterning. It plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. It is also shown to be involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Its DNA-binding region is localized to amino acids 278-458. Conditional inactivation of TBR-2 during early brain development is known to cause microcephaly and severe behavior deficits. Two isoforms of TBR-2 have been described that are produced by alternative splicing. Isoform 2 lacks amino acids 463-481. (Ref.: Bulfone, A., et al. (1999). Mech. Dev. 84 (1-2); 133-138).

Specificity

This rabbit polyclonal antibody detects T-box brain protein 2 (TBR-2) in murine species. It targets an epitope with in 20 amino acids from the C-terminal region.

Immunogen

Conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine TBR-2.

Epitope: C-terminus

Application

Anti-TBR-2, Cat. No. ABN1687, is a highly specific rabbit polyclonal antibody that targets Eomesodermin homolog (Tbr2) and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected TBR-2 in Immunofluorescence applications (Nelson, B.R., et. al. (2013). J Neurosci. 33(21):9122-39; Hodge, R.D., et. al. (2013). J Neurosci. 33(9):4165-80).

Immunohistochemistry Analysis: A representative lot detected TBR-2 in Immunohistochemistry applications (Yoon, K.J., et. al. (2008). Neuron. 58(4):519-31).

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected TBR-2 in mouse hippocampus, embryonic mouse brain, and embryonic mouse gut tissue sections.

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in mouse E13-14 brain.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBR-2 in mouse E13-14 brain.

Target description

~63 kDa observed; 74.80 kDa calculated.

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. species reactivity (predicted by homology): rat (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. g. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. isotype: IgG. NCBI accession no.: NP_034266. UniProt accession no.: O54839. target post-translational modification: unmodified. Gene Information: mouse ... Eomes(13813).