General description
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
Specificity
This antibody recognizes TCF-4
Immunogen
Amino acids 31-331 of human TCF-4.
Application
Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).
Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).
Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.
Quality
Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).
Target description
Mr 66 kDa
Linkage
Replaces: 04-1080
Physical form
Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Analysis Note
Control
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51171984
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 05-511
- Temperature Control Device:
- Yes
