Anti-Tentonin 3/TMEM150c (C15-1317-504)

General description

Transmembrane protein 150C (UniProt: Q8C8S3; also known as Tentonin, TTN3) is encoded by the Tmem150c gene (Gene ID: 231503) in murine species. TTN3 is a multi-pass membrane protein of the DRAM/TMEM150 family. Two isoforms of TTN3 have been described that are produced by alternative splicing. It belongs to a new class of mechanotransduction channel that is expressed in muscle spindle afferents and contribute to proprioception (touch sensation). It is activated by mechanical stimuli with distinctly slow inactivation kinetics. Mice with deficiency in TTN3 display loss of coordinated movements and abnormal gait. It is reported to mediate slow adapting currents in DRG neurons.

Specificity

This rabbit polyclonal antibody detects Transmembrane protein 150C in mouse and rat cells. it targets an epitope within 38 amino acids from the C-terminal region.

Immunogen

A linear peptide corresponding to 38 amino acids from the C-terminal region of murine Transmembrane protein 150C.

Epitope: C-terminus

Application

Anti-Tentonin 3/TMEM150c, Cat. No. ABN2266, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 150C and has been tested in , Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in muscle spindle from mouse (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).

Immunocytochemistry Analysis: A representative lot detected Tentonin 3/TMEM150c in HEK293T cells transfected with TTN3. (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).

Western Blotting Analysis: A representative lot detected Tentonin 3/TMEM150c in F11 rat DRG cells (untransfected and TTN3 transfected cells) (Hong, G.S., et. al. (2016). Neuron. 91(1):107-18).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in F11 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Tentonin 3/TMEM150c in 10 µg of F11 cell lysate.

Target description

~25 kDa observed; 27.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, rat. packaging: antibody small pack of 25 . μ. L. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_878261. UniProt accession no.: Q8C8S3. shipped in: ambient. target post-translational modification: unmodified. Gene Information: mouse ... Tmem150C(231503).