ANTI-TFG ANTIBODY (C15-1317-464)

General description

The TRK-fused gene protein (TFG) gene is highly conserved among mammalian species, and is widely studied as a fusion gene partnering with various oncogenes. The function of the resultant TFG protein product is still unclear. TFG regulates the activity of phosphotyrosine-specific phosphatase-1 (SHP-1), and may be important in cells containing SHP-1 such as epithelial and neuronal cells. Defective TFG contributes to thyroid papillary carcinoma.

Specificity

This antibody recognizes the C-terminus of TFG.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human TFG.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-TFG Antibody is validated for use in Western Blotting, IHC(P) for the detection of TFG.

Western Blot Analysis: 0.1 µg/mL from a representative lot detected TFG on 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFG in mouse hippocampus, mouse cerebellum, human placenta, and human pancreas tissues.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected TFG on 10 µg of HeLa cell lysate.

Target description

~56 kDa observed. The calculated molecular weight is 43 kDa, however TFG has been shown as a ~56 kDa band in western blots (Greco, A., et al. (1995). Molecular and Cellular Biology. 15(11):6118–6127.)

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse, human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_001007566. UniProt accession no.: Q92734. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TFG(10342). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.