Anti-Tie2 Mouse mAb (Ab33) (C15-1307-028)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.

Recognizes the ~140-45 kDa Tie2 protein.

This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Immunogen

Human

a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag sequence

Application

Frozen Sections (see comments)

Immunoblotting (0.2-1 µg/ml, chemiluminescence)

Immunoprecipitation (see comments)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For frozen sections, tissues were treated with Triton^™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: Ab33, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: human, porcine, mouse, rat. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG1. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... TEK(7010). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.