General description

The 1G9 antibody reacts with mouse TIGIT (T cell Ig and ITIM domain), a 26 kDa member of the CD28 receptor family which is reported to regulate T cell receptor (TCR) activation. Within the CD28 family of receptors there are those which have co-stimulatory activity, such as CD28 and CTLA-4, as well as more recently identified receptors like TIGIT which are proposed to provide co-inhibitory signals. TIGIT is expressed and upregulated on activated T cells, and is also expressed on memory and regulatory T cells. Upon engagement by its ligands, CD112 and CD155, TIGIT signaling inhibits T cell proliferation and suppresses T cell responses, without triggering cell deletion. A second inhibitory effect of TIGIT signaling is the generation of immunoregulatory dendritic cells, which secrete IL-10 and TGF-beta to further inhibit T cell function.The 1G9 antibody may be used for flow cytometric analysis of TIGIT, which is expressed at very high levels on T regulatory cells (Tregs) and activated conventional T cells, as well as memory T cells and NK cells.

Anti-TIGIT (mouse), PE, clone 1G9 Antibody is an antibody against TIGIT for use in Flow Cytometry.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected TIGIT in one million C57Bl/6 splenocytes.