General description
As a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. It is known to synergize with Toll-like receptors and is thought to regulate Th1 and Th17 cytokine secretion.
Immunogen
Recombinant protein corresponding to human Tim3.
Application
Anti-Tim3 Antibody, clone 2E2 detects level of Tim3 & has been published & validated for use in WB, FC.
Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected Tim3 in human monocyte derived dendritic cells.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Quality
Evaluated by Western Blot in human thymus tissue lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Tim3 in human thymus tissue lysate.
Target description
~30 kDa observed.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human thymus tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51201631
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABF62
- Temperature Control Device:
- Yes
