Anti-TMEM100 (C15-1317-452)

General description

Transmembrane protein 100 (UniProt: Q9CQG9; also known as TMEM100) is encoded by the Tmem100 gene (Gene ID: 67888) in murine species. TMEM100 is a multi-pass membrane protein that is shown to be involved in renal development, vasculogenesis and lung cancer invasiveness. It plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. TMEM100 is also involved in the regulation of nociception by forming a complex with TRPA1 and TRPV1 and act as a modulator of their interaction in dorsal root ganglia (DRG) neurons. It is reported to weaken TRPA1 and TRPV1 interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex. TMEM100-deficient mice show a reduction in inflammatory mechanical hyperalgesia and TRPA1- but not TRPV1-mediated pain. A mutation involving aa 117-119 KRR to QQQ is shown to abolish TMEM100 interaction with TRPA1, but increases interaction with TRPV1. Mutations is Tmem100 gene can lead to embryonic lethality due to impaired differentiation of arterial endothelium and defects of vascular morphogenesis. (Ref.: Weng, H-J., et al. (2015). Neuron 85(4), 833-846).

Specificity

This rabbit polyclonal antibody detects transmembrane protein 100 (TMEM100) in murine cells. It targets an epitope with the 19 amino acids from the N-terminal end.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminus region of murine Transmembrane protein 100 (TMEM100).

Application

Anti-TMEM100, Cat. No. ABN1721, is a highly specific rabbit polyclonal antibody that targets Transmembrane protein 100 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected TMEM100 in mouse colon, rat peripheral nerve, and human cerebral cortex tissues.

Immunofluorescence Analysis: A representative lot detected TMEM100 in DRGs (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).

Western Blotting Analysis: A representative lot detected TMEM100 in DRG from cervical to lumbar levels (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).

Immunoprecipitation Analysis: representative lot detected TMEM100 in whole-cell DRG or CHO cells lysates (Weng, H.J., et. al. (2015). Neuron. 85(4):833-46).

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) of WT & TMEM100 CKO mice (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).

Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected TMEM100 in Dorsal root ganglion (DRG) sections from Avil-Cre;Tmem100+/+ (Wild Type mice) and Avil-Cre;Tmem100fl/fl (Tmem100 CKO) mice (Courtesy of Drs. Hao-Jui Weng and Xinzhong Dong at Johns Hopkins University).

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TMEM100 in 10 µg of mouse lung tissue lysate.

Target description

~17 kDa observed; 14.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. packaging: antibody small pack of 25 . μ. g. technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_080709. UniProt accession no.: Q9CQG9. target post-translational modification: unmodified. Gene Information: mouse ... Tmem100(67888). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.