General description
Anoctamin-2 (UniProt: Q8CFW1; also known as Transmembrane protein 16B, TMEM16B) is encoded by the Ano2 (also known as Tmem16b) gene (Gene ID: 243634) in murine species. TMEM16B is a multi-pass membrane protein that serves as a calcium-activated chloride channel (CaCC) that plays a role in olfactory signal transduction and also mediates light perception amplification in retina. Its channel activity is shown to be repressed by chloride channel inhibitors, such as niflumic acid and flufenaic acid. TMEM16 B is expressed in retina, especially in the photoreceptor synaptic terminals, and in olfactory epithelium, particularly in sensory neurons (OSNs) and cilia. It is also shown to be expressed in retinal pigment epithelium (RPE), olfactory bulb, and cortex. It is detected in the mantle layer of the neural tube and in the dorsal root ganglia at E14.5. TMEM16B is also involved in a calcium-dependent mode of ionic plasticity that reduces the efficacy of GABAergic synapses. It is reported to attenuate GABAergic transmission by increasing the postsynaptic chloride concentration, hence reducing the driving force for chloride influx.
Specificity
Clone 13B8 detects Transmembrane protein 16B (TMEM16B) in murine neural tissue.
Immunogen
GST-tagged recombinant fragment corresponding to the first 74 amino acids of murine Transmembrane protein 16B.
Application
Anti-TMEM16B, clone 13B8, Cat. No. MABN2433, is a highly specific mouse monoclonal antibody that targets Transmembrane protein 16B (Anoctamin-2) and has been tested in Immunofluorescence and Western Blotting.
Western Blotting Analysis: A representative lot detected TMEM16B in mouse retina tissue (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).
Immunofluorescence Analysis: A representative lot detected TMEM16B in mouse retina tissue (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).
Quality
Evaluated by Immunohistochemistry in mouse retinal tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected TMEM16B in mouse retinal tissue.
Target description
130 kDa observed; 114.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
- UPC:
- 41116126
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- MABN2433
- Product Size:
- 100/µG
