General description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Recognizes recombinant and monocyte-derived TNF-α. Exhibits some cross-reactivity with lymphotoxin (TNF-β) and mouse TNF-α in L929 cells.
This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.
Immunogen
recombinant, human TNF-α
Application
ELISA (see comments)
Frozen Sections (see comments)
Immunoblotting (see comments)
Neutralization Studies (see comments)
Paraffin Sections (see comments)
Radioimmunoassay (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
Analysis Note
Positive Control
L929 cells
Other Notes
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity: human, mouse (weakly). manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. impurities: ≤. 1 . EU/mg Endotoxins. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: human ... TNF(7124). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202416
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 654250-1MG