ANTI-TOE1 (AB-86) (C005B-241067)

General description

Target of EGR1 protein 1 (UniProt Q96GM8) is encoded by the TOE1 gene (Gene ID 114034) in human. Originally characterized and thereby named as a downstream target of the immediate early gene Egr1, TOE1 is a nuclear protein localized primarily in nucleoli and Cajal bodies. TOE1 overexpression causes an upregulation of the cyclin-dependent kinase inhibitor p21 and cell cycle arrest at G2/M. TOE1 also interacts wtih p53 C-terminal tetramerization domain and enhances p53 transcriptional activity. In addtion, TOE1 exhibits inhibitory activity against HIV-1 replication and evidence indicates TOE1 interaction with the viral specific transactivator response element as part of the inhibitory mechanism. TOE1 is upregulated and secreted by activated CD8(+) T lymphocytes, and TOE1 can be proeolytically cleaved by granzyme B at D328 and D363, generating a 35-amino acid (a.a. 329-363) fragment containing the nuclear localization signal/NLS (a.a.335-347). The 35-a.a. fragment retains both the antiviral potency and cell-penetrating capability. Human TOE1 is produced as a 510-a.a. protein, containing a C3H1-type Zinc finger (a.a. 294-322) and anuclear localization signal/NLS (a.a.335-347). The initiation methionine is removed posttranslationally.

Specificity

This polyclonal antibody was raised against the C-terminal end sequence present in both spliced isoforms of human TOE1 reported by UniProt (Q96GM8).

Immunogen

Conjugated linear peptide corresponding to the C-terminal end sequence of human TOE1.

Epitope: C-terminus

Application

Immunoprecipitation Analysis: 1 µL from a representative lot immunoprecipitated TOE1 from 20 µg of HEK293T cell lysate (Courtesy of Dr. Ian de Belle).

Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type or mutant TOE1 with D328A, D363A/D373A, or D387A point mutions following their immunoprecipitation by an anti-FLAG antibody (Sperandio, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(26):E3392-E3401).

Research Category
Inflammation & Immunology

This rabbit polyclonal Anti-TOE1 (Ab-86) Antibody, Cat. No. ABF1069 is validated for use in Immunoprecipitation and Western Blotting for the detection of TOE1.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TOE1 in 10 µg of HeLa cell lysate.

Target description

~58 kDa observed. 56.42/47.76 kDa (isoform 1/2) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_079353. UniProt accession no.: Q96GM8. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... TOE1(114034). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.