Anti-TorsinB Antibody (C15-1316-589)

Specificity

TorsinB. The antibody reacts with the ~38 kDa protein.

Immunogen

Synthetic peptide from torsinB.

Application

Detect TorsinB using this Anti-TorsinB Antibody validated for use in IP, WB & IC.

Western blot: 1:100-1:200. The antibody reacts with the ~38 kDa protein. The suggested blocking buffer is TBS with 10% milk and 0.05% Tween-20. The suggested dilution buffer is TBS with 1% milk and 0.05% Tween-20. Note: TorsinB is a glycoprotein which can be deglycosylated by either EndoH or PNGase treatment.
Immunocytochemistry: 1:100 using rhodamine conjugated goat anti-rabbit secondary antibody. Suggested fixative is 4% paraformaldehyde fixed (37°C for 20 minutes) cells. Suggested blocking solution should contain 10% goat serum. Suggested dilution buffer is PBS containing 1% BSA.
Immunoprecipitation. Recommended antibody amount is 5-7 mL of rabbit serum. The preferred tissue/cell lysis buffer is RIPA. Final reaction volume of 1000 mL. Final total protein concentration in reaction mix: 0.1-0.5 mg/mL. Suggested incubation time is 16-18 hours at 2-8°C. Sugggested capture is Protein A/G.

Optimal working dilutions must be determined by end user.

Storage and Stability

Maintain at frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Western blot: brain and liver. Immunocytochemistry: SH-SY5Y cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse, human. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_014506.1. UniProt accession no.: O14657. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... TOR1B(27348). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.