ANTI-TRAF3 (C005B-241044)

General description

TNF receptor-associated factor 3 (UniProt Q13114; also known as CAP-1, CD40 receptor-associated factor 1, CD40-binding protein, CD40BP, CRAF1, LAP1, LMP1-associated protein 1) is encoded by the TRAF3 (also known as CAP1, CRAF1) gene (Gene ID 7187) in human. NF-κB-inducing kinase (NIK) is negatively regulated by a complex composed of the E3 ubiquitin ligases c-IAP1 and 2 (cellular inhibitor of apoptosis 1 and 2) and the adaptors TRAF2 and 3 (TNF receptor-associated factors 2 and 3). The TRAF2-TRAF3 complex bridges the c-IAPs and NIK into close proximity, where TRAF2 recruits c-IAP1/2 and TRAF3 binds to NIK. NF-κB signaling activation results in TRAF3 degradation, leading to upregulated NIK. TRAF3 deletion in B-cells results in uncontrolled growth and TRAF3 mutations have been identified in several human B-cell cancers, including Hodgkin lymphoma, splenic marginal zone lymphoma, and multiple myeloma. TRAF3 also plays an indirect role in promoting PI3K/AKT and JAK/STAT signalings, which are negatively regulated by NIK. TRAF3 prevents NIK from targeting PTEN to the plasma membrane and thereby promoting PI3K/AKT signaling. TRAF3 is required for normal T cells to function properly and anaplastic large cell lymphoma (ALCL) cells reply on PI3K/AKT and JAK/STAT signaling for proliferation. Opposite to B-cell cancers, TRAF3 knockdown triggers cell cycle arrest in ALCL cells. Human TRAF3 contains one RING-type (a.a. 68-77) and two TRAF-type (a.a. 135-190 and 191-249) zinc fingers, a , coiled coil (a.a. 267-338) regin, followed a C-terminal MATH (meprin and TRAF homology) domain (a.a. 415-560) that mediates interaction with receptor cytomplasmic domain.

Specificity

This rabbit polyclonal antibody targets a coiled coil region sequence conserved among bovine, equine, human, mouse, and rat TRAF3 protein sequences.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence within the coiled coil region of human TRAF3

Application

Anti-TRAF3 Antibody, Cat. No. ABE861, is a highly specific rabbit polyclonal antibody that targets TNF receptor-associated factor 3 (TRAF3) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRAF3 in rat testis as well as in human kidney and pancreas tissue sections.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TRAF3 in 10 µg of Jurkat cell lysate.

Target description

~64 kDa observed. 64.49/55.31 kDa (human isoform 1/2), 64.29/64.19 kDa (mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: ABE585

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat. species reactivity (predicted by homology): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_003291. UniProt accession no.: Q13114. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... CAP1(10487), TRAF3(7187). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.