Anti-Transcription Initiation Factor IIA subunit 1 (C15-1316-952)

General description

Transcription initiation factor IIA subunit 1 (UniProt: P52655; also known as General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42) is encoded by the GTF2A1 (also known as TF2A1) gene (Gene ID: 2957) in human. TFIIA is one of the several components of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation. TFIIA in a complex with TATA-box-binding protein (TBP) mediates transcriptional activity. After initiation, some components of the pre-initiation complex, including TFIIA, remain attached and re-initiate a subsequent round of transcription. Binding of TFIIA to TBP helps to stabilize TBP binding to the TATA element. TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12).

Specificity

This rabbit polyclonal antibody detects Transcription initiation factor IIA subunit 1 in human cells. It targets an epitope within 15 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Transcription initiation factor IIA subunit 1.

Application

Anti-Transcription Initiation Factor IIA subunit 1, Cat. No. ABE1797, is a highly specific rabbit polyclonal antibody that targets Transcription initiation factor IIA subunit 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Transcription initiation factor IIA subunit 1 in 10 µg of RAW264.7 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Transcription initiation factor IIA subunit 1 in human brain and human testis tissue.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Transcription initiation factor IIA subunit 1 in 10 µg of HeLa cell lysate.

Target description

~55 kDa observed; 41.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. isotype: IgG. NCBI accession no.: NP_056943. UniProt accession no.: P52655. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... GTF2A1(2957).