ANTI-TRANSIENT RECEPTOR POTENTIAL CHANNEL 7 ANTIBODY (C005B-239636)

Specificity

Recognizes Transient Receptor Potential Channel 7 (TRPM7, LTRPC7, Channel-kinase 1).

Immunogen

Purified peptide from the C-terminus of human TRPM7 (Accession number Q96QT4).

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Transient Receptor Potential Channel 7 Antibody is validated for use in IC, WB for the detection of Transient Receptor Potential Channel 7.

Western blot: 1:200-1:500 using ECL on mouse brain lysate and GH3 cell line (rat pituitary tumor) lysate.
Immunocytochemistry: 1:200 using GH3 cell line (rat pituitary tumor).
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

ImmunoAffinity Purified

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 200 uL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NM_017672.3. UniProt accession no.: Q96QT4. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Trpm7(58800)
rat ... Trpm7(679906). Pictograms: GHS06,GHS09. Signal Word: Danger. Hazard Statements: H301 + H311 + H331 - H411. Precautionary Statements: P261 - P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311. Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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