Skip to main content

ANTI-TREM-2 ECTODOMAIN CLONE 5F4 (C15-1886-148)

Sigma Aldrich

Catalog No.
C15-1886-148
Manufacturer No.
MABN2322-100UL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
EA
Price: $720.37
List Price: $800.42

General description Beta-nerve growth factor (UniProt: P01139: also known as Beta-NGF) is encoded by the Ngf (also known as Ngfb) gene (Gene ID: 18049) in murine species. β-NGF, an endogenous neurotrophic factor, is a homodimeric glycoprotein

Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. Contact Us

Adding to cart… The item has been added

General description

Beta-nerve growth factor (UniProt: P01139; also known as Beta-NGF) is encoded by the Ngf (also known as Ngfb) gene (Gene ID: 18049) in murine species. β-NGF, an endogenous neurotrophic factor, is a homodimeric glycoprotein that is synthesized with a signal peptide (aa 1-18) and a propeptide (aa 19-121) that are subsequently cleaved off to generate the mature form (aa 122-241). It is highly expressed in male submaxillary gland and its levels are much lower in female submaxillary gland. Its expression is reported to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain. NGF is important for the development and maintenance of the sympathetic and sensory nervous systems. It serves as an extracellular ligand for the NTRK1 and NGF receptors to activate cellular signaling cascades to regulate neuronal proliferation, differentiation, and survival. However, it does not permeate the blood-brain barrier. Its precursor form (ProNGF) is reported to function as a ligand for the heterodimeric receptor formed by Sortilin Related VPS10 Domain Containing Receptor 2 (SORCS2) and NGF receptor that activates signaling cascade leading to inactivation of RAC1 and/or RAC2. In contrast to the mature form, ProNGF is known to promote neuronal apoptosis. N-glycosylation of ProNGF is reported to be essential for its processing, secretion, and construction of the tertiary fold of the β-NGF homodimer. Clone D11 is a potent antagonist that can effectively antagonize rodent and human NGF in a variety of in vitro and in vivo systems. (Ref.: Covaceuszach, S., et al. (2012). PLoS ONE 7(3): e32212; Feng, D., et al. (2010). J. Mol. Biol. 396(4); 967-984; Scott, J., et al. (1983). Nature. 302(5908); 538-540).

Specificity

Clone D11 is a rat monoclonal antibody that detects Nerve growth factor (NGF). It targets an epitope within 23 amino acids from the N-terminal half of the mature NGF.

Immunogen

2.5S NGF purified from the mouse submandibular glands.

Application

Quality Control Testing

Evaluated by ELISA with recombinant mouse Nerve growth factor.

ELISA Analysis (ELISA): A representative lot of this antibody detected various dilution of recombinant mouse Nerve growth factor.

Tested applications

Surface plasmon resonance: A representative lot detected Nerve Growth Factor in Surface plasmon resonance applications (Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

ELISA Analysis: A representative lot detected Nerve Growth Factor in ELISA applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10; Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

Inhibition Assay: A representative lot competitively inhibited the biological activity of mNGF and prevented the binding of NGF to PC12 cells. (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Radioimmunoassay: A representative lot detected Nerve growth factor in Radioimmunoassay applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Nerve Growth Factor, clone D11, Cat. No. MABN2322, is a rat monoclonal antibody that detects Nerve growth factor and is tested for use in ELISA, Inhibition Assay, Radioimmunoassay, and Surface Plasmon Resonance.

Physical form

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

UPC:
41106618
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
MABN2322-100UL
Product Size:
100/µL

Cenmed Satisfaction Guarantee
Cenmed Satisfaction Guarantee

At Cenmed, your confidence and satisfaction are paramount. We guarantee the quality and reliability of our extensive range of clinical and laboratory supplies. If you're not completely satisfied with your purchase, we offer a straightforward return process and dedicated support to resolve your concerns promptly. Our commitment ensures that you can order with confidence, knowing that Cenmed is dedicated to superior service and customer satisfaction. Trust us to meet your needs with every order, backed by our promise of excellence. Learn more in Help & FAQs.

Testimonial
"Cenmed provides me access to the same products/services normally reserved for much larger labs than mine. I was presently surprised by their product offering."

LAB DIRECTOR

Testimonial
"We utilized Cenmed's capabilities for a variety of projects around the world. They are a valued partner and supplier."

PHARMACEUTICAL SUPPLY CHAIN LEADER

Testimonial
"The reps are very good at finding products for customers in this period of supply chain issues."

SCOTT BEHMAN

Testimonial
"Your customer service has been excellent and makes me excited about purchasing with Cenmed in the future!!"

PROCUREMENT + BILLING COORDINATOR AT PHARMA.