General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
Specificity
The unconjugated parent antibody of this antibody is Cat. No. 07-449.
Immunogen
Branch peptide corresponding to trimethyl-Histone H3 (Lys27).
Application
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
This Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies
Target description
~17 kDa observed. The unconjugated parent antibody (Cat. No. 07-449) has an observed molecular weight at ~17 kDa
Physical form
Protein A purified
Purified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51172414
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 07-449-AF555
- Temperature Control Device:
- Yes
