Anti-trimethyl STAT3 (Lys180) Antibody (C15-1316-912)

General description

Signal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 is known to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). STAT3 gene knockout in mice is lethal at the early embryonic stage.

Specificity

Trimethylation site is present in all three human spliced isoforms. Reactivity toward Stat3 with mono- or dimethylated Lys180 has not been tested.

Immunogen

Epitope: STAT3 K180me3

KLH-conjugated linear peptide corresponding to the STAT3 K180me3 of human trimethyl STAT3 (Lys180)

Application

Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated STAT3 trimethylated on Lys180 in 0.5 mg of mouse embryonic stem cell lysate.
Peptide Inhibition Assay: Target specificity of a representative lot was confirmed by immunogen peptide blocking prior to Western blotting analysis (0.5 µg/mL) ) of mouse embryonic stem cell lysate (10 µg/lane) . Only the immunogen peptide, but not the corresponding peptide without the Lys180 trimethylation modification, blocked the detection of the target band.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-trimethyl STAT3 (Lys180) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay, Immunoprecipitation for the detection of trimethyl STAT3 (Lys180).

Quality

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAT3 trimethylated on Lys180 in 10 µg of mouse embryonic stem cell lysate.

Target description

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. species reactivity (predicted by homology): human (based on 100% sequence homology), porcine (based on 100% sequence homology), orangutan (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology). technique(s): immunoprecipitation (IP): suitable, inhibition assay: suitable (peptide), western blot: suitable. NCBI accession no.: NP_644805. UniProt accession no.: P40763. shipped in: wet ice. target post-translational modification: trimethylation (Lys180 ). Gene Information: human ... STAT3(6774). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.