ANTI-TROPOMYOSIN ANTIBODY CLONE 15D12.2

General description

Tropomyosins (Tms) are a family of >40 highly conserved protein isoforms resulting from alternative splicing of four different genes during development and that are expressed in numerous cell types. These proteins form coiled-coil heterodimers which bind to actin polymers along the α-helical grove. Tropomyosin’s function varies depending on cell type in which it is expressed. Within muscle, Tms play a role in the regulation of actin-myosin interplay for the purpose of mediating muscle contraction. In non-muscle cells, studies suggest a role in the stabilization of actin filaments, along with protecting actin filaments from the severing action of gelsolin and ADF/cofilin depolymerization. Tms also appear to have a role in the moderation of access to actin for other actin-binding proteins. Tropomyosin has an integral role in maintaining cardiovascular homeostasis. Down-regulation of Tms expression has been observed in tumor cells indicating a possible role for Tms as tumor suppressors.

Specificity

The antibody recognizes the entire exon 9d of Tropomyosin.

Immunogen

Epitope: Exon 9d

Linear peptide corresponding to the entire exon 9d of rat Tropomyosin.

Application

Detect Tropomyosin using this Anti-Tropomyosin Antibody, clone 15D12.2 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected Tropomyosin in human striated muscle tissue.

Research Category
Cell Structure

Metabolism

Research Sub Category
Cytoskeleton

Muscle Physiology

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.

Target description

~ 30-40 kDa observed MWs. Tm6: 40 kDa; Tm1: 38 kDa; Tm2: 36 kDa; Tm3: 34 kDa; Tm5: 30 kDa

Physical form

Format: Purified

Protein G

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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