Anti-Tubulin Antibody, Detyrosinated (C15-1316-030)

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Specificity

Expected to cross-react with mammals, sea urchin, plants, not yeast (Paturle-Lafanechere, L., 1991).

The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).

Immunogen

Peptide corresponding to the C-terminal of Glu tubulin.

Application

Anti-Tubulin Antibody, Detyrosinated is an antibody against Tubulin for use in IC, IH(P) and WB.

Immunohistochemistry:
A previous lot was used on either frozen or paraffin embedded, fixed tissue (Gundersen, G.G., 1984): 1:200-1:500 dilution.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by Western Blot on L6 lysate lysates.

Western Blot Analysis: 1:500 dilution of this lot detected detyrosinated tubulin on 10 μg of L6 lysate.

Target description

50 kDa

Physical form

Affinity purified immunoglobulin

Purified rabbit polyclonal in buffer containing liquid PBS.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Hela Whole Cell Lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse, sea urchin, human, plant. should not react with : Saccharomyces cerevisiae. species reactivity (predicted by homology): mammals. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NM_006086.2. UniProt accession no.: Q13509. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... TUBA1A(7846). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.