General description
Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.
Specificity
This antibody reacts with the C-terminus of the beta-III isoform of tubulin, which is known to be neuron specific. Does not identify beta tubulin found in glial cells.
Immunogen
Epitope: C-terminus
Synthetic peptide corresponding to human Tubulin β-III.
Application
Detect Tubulin β-III using this Anti-Tubulin β-III Antibody, clone TU-20, Alexa Fluor™555 Conjugate validated for use in ICC.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Quality
Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tubulin β-III in rat E18 primary cortex cells.
Target description
The unconjugated parent antibody (Cat. No. MAB1637) has an observed molecular weight at ~55 kDa
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor™ 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Rat E18 primary cortex cells
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352202
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- CBL412A5
- Temperature Control Device:
- Yes
