Specificity
Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
Immunogen
Highly pure (>98%) recombinant Mouse TNF-alpha.
Application
Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB.
ELISA: A concentration of at least 0.5μg/mL (100μL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha.
Western Blot: A concentration of 0.1-0.2 μg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 μg/ml of AB2148P is required.
Optimal working dilutions must be determined by the end user
Linkage
Replaces: AB1450P
Physical form
Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, neutralization: suitable, western blot: suitable. NCBI accession no.: NP_038721. UniProt accession no.: P06804. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... TNF(7124). Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB2148P
