Anti-Tyrosine Hydroxylase Rabbit pAb (C15-1307-389)

General description

Affinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa tyrosine hydroxylase protein.

Recognizes the ~60 kDa tyrosine hydroxylase protein in okadaic acid-treated PC12 cells.

This Anti-Tyrosine Hydroxylase Rabbit pAb is validated for use in Dot Blot, Immunoblotting, Immunofluorescence, Frozen Sections for the detection of Tyrosine Hydroxylase.

Immunogen

Rat

purified, SDS-denatured rat pheochromocytoma tyrosine hydroxylase

Application

Dot Blot (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:1000)

Frozen Sections (1:1000; see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM HEPES, 50% glycerol, 0.01% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Cross-reacts with most mammalian forms and some non-mammalian forms of tyrosine hydroxylase. Variables associated with assay conditions will dictate the proper working dilution.

Xu, Z.Q. 1998. Neurosci.82, 727.
Haycock, J.W. 1993. Anal. Biochem.208, 397.
Lewis, D.A., et al. 1993. Neuroscience54, 477.
Haycock, J. 1989. Anal. Biochem.181, 259.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity (predicted by homology): all. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.