ANTI-UGT1A9 CLONE 14G11 25 MIG (C005B-448846)

Specificity

Clone 18C8 is a is a mouse monoclonal antibody that detects Syntaxin-5. It targets an epitope within the cytoplasmic domain in the C-terminal half. It detects both isoform 1 and 2.

Immunogen

GST-tagged recombinant fragment corresponding to 83 amino acids from the C-terminal half, SNARE motif of rat.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue extract and NRK cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Syntaxin-5 in Rat brain tissue extract and normal rat kidney epithelial (NRK) cell lysate.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated Syntaxin 5 in Immunoprecipitation applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Immunocytochemistry Analysis: A representative lot detected Syntaxin 5 in Immunocytochemistry applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Western Blotting Analysis: A representative lot detected Syntaxin 5 in Western Blotting applications (Williams, A.L., et al. (2004). Mol Biol Cell. 15(1):162-75).

Inhibition Assay: A representative lot inhibited the formation of a ternary SNARE complex. (Williams, A.L., et al. (2004). Mol Biol Cell.;15(1):162-75).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Syntaxin-5 (UniProt: Q08851; also known as STX-5) is encoded by the Stx5 (also known as Stx5a) gene (Gene ID: 65134) in rat. . Syntaxin-5 is a single-pass type IV membrane protein that localizes throughout the Golgi apparatus and is most abundant in the cis-most cisternae. It is found in the endoplasmic reticulum (ER)-Golgi intermediate compartment membrane. It has a cytoplasmic domain (aa 1-333), a transmembrane domain (aa 334-354), and a single amino acid vesicular domain (aa 355). Its expression has been reported in the brain, heart, spleen, lung, liver, kidney, and testis. It mediates ER to Golgi transport and together with vesicular transport factor p115 (USO1) and Golgi matrix protein GM130 (GOLGA2), it is involved in tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Syntaxin-5 is shown to protect cells against proteotoxicity and is involved in clearance of insoluble protein deposits. Clone 18C8 is shown to bind to syntaxin 5 SNARE motif and inhibit SNARE complex formation and prevents the SNARE motif from engaging other SNAREs. It binds to syntaxin 5 molecules with unengaged, SNARE motifs. (Ref.: Babazadeh, R., et al. (2019). Cell Rep. 28(8); 2096-2110; Diao, A., et al (2008); J. Biol. Chem. 283(11); 6957-6967; Williams, AL., et al. (2004). Mol. Biol. Cell. 15; 162-175).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.