ANTI-ULBP-3 CLONE 3C10 ZOOMAB« RABBIT MONOCLONAL (C005B-262465)

General description

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ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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Specificity

Clone 3C10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects UL16-binding protein-3 (ULBP-3). It targets an epitope within 21 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of mature human U16-binding protein 3 (ULBP-3).

Application

Anti-ULBP-3, clone 3C10 ZooMAb, Cat. No. ZRB1033, is a recombinant Rabbit monoclonal antibody that specifically targets ULBP-3 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ULBP-3 in NTERA-2 cells.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected ULBP-3 in NTERA-2 cells.

Affinity Binding Assay: A representative lot of this antibody bound ULBP-3 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

UL16-binding protein 3 (UniProt: Q9BZM4; also known as ALCAN-gamma, NKG2D ligand 3, N2DL-3, NKG2DL3, Retinoic acid early transcript 1N, ULBP3) is encoded by the ULBP3 (also known as N2DL3, RAET1N) gene (Gene ID: 79465) in human. ULBPs are GPI-linked glycoproteins that belong to the extended MHC class I family. They are known to stimulate cytokine and chemokine production from NK cells. ULBP3 is a GPI-anchor protein that binds and activates NKG2D receptor, mediating natural killer (NK) cell cytotoxicity. It is synthesized with a signal peptide (aa 1-29) and a propeptide (aa 218-244), that are subsequently cleaved off to produce the mature form (aa 30-217). The domain structure of ULBP3 differs from other conventional MHC class I molecules in that it does not contain the alpha 3 domain and the transmembrane segment. It is composed of only the alpha 1-alpha2 domain. Overexpression of ULBP3 has been reported in several tumor cell lines and serum samples from tumor subjects contain elevated levels of soluble form of ULBP3. Elevated levels of soluble ULBP-3 can induce protein tyrosine phosphorylation and activation of several protein kinases, including JAK2, MAP kinases, and PI3-kinase/Akt. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mou, X., et al (2015). Sci. Rep. 4; Article 6138).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.