ANTI-VASOHIBIN-1 ANTIBODY CLONE 4E12 (AZIDE FREE)

General description

Vasohibin-1 is an endothelium-derived negative feedback regulator of angiogenesis. Vasohibin-1 inhibited migration, proliferation, and network formation by ECs in culture, as well as angiogenesis in vivo, and Vasohibin-1 was selective for endothelial cells and its influence was eliminated by hypoxia or TNF-alpha interaction. Vasohibin is preferentially expressed in endothelial cells and does not appear to promote proliferation or tumor growth when over expressed. Vasohibin-1 expression is induced by VEGF expression and its effects are mediated through VEGF-R2 independent of VEGF-R1 and Vasohibin-1 may directly influence the expression of VEGF-R2. Because the inhibition effects of Vasohibin-1 are nullified by hypoxia and various cytokines it is thought that this is how tumors escape this built-in negative regulator of angiogenesis. Vasohibin-1 is highly expressed in fetal organs and the endothelial cells during development, and also expressed in the brain and placental and microvessels endothelial cells of atherosclerotic lesions in the adult.

Immunogen

KLH-conjugated linear peptide corresponding to Human Vasohibin-1.

Application

Functional Activity Assay: A representative lot of this antibody induced cellular senescence (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).

Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 in HUVECs transfected with control siRNA, but not in HUVECs transfected with Vasohibin-1 siRNA (Miyashita, H., et al. (2012) PLOS ONE. 7(10):e46459).

Western Blotting Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in VEGF-stimulated HUVECs and GM7373 transfected cell lysate (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Immunohistochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in human placenta tissue (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Immunocytochemistry Analysis: A representative lot of this antibody detected Vasohibin-1 (KIAA1036 protein referenced in publication) in HUVEC cells (Watanabe, K,, et al. (2004) JCI. 114(7):898-907).

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-Vasohibin-1 Antibody, clone 4E12 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry and Activity Assay for the detection of Vasohibin-1.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Vasohibin-1 in 10 µg of HEK293 cell lysate.

Target description

~ 41 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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