ANTI-VEGF-A (RABBIT POLYCLONAL) (C15-1315-909)

General description

The human VEGFA, a 45 kDa homodimeric glycoprotein, is one of the most potent angiogenic factors involved in endothelial cell proliferation, migration, and secretion of MMPs. It also serves as an important survival factor for endothelial cells, inhibiting apoptosis. VEGFA belongs to a family that includes VEGFA, VEGFB, VEGFC, VEGFD, and placental growth factor (PLGF). In humans four prominent isoforms of VEGFA are recognized, VEGF121, VEGF165, VEGF189, and VEGF206. In addition, two other less frequent splice variants, VEGF145 and VEGF183, have also been reported. The properties of native VEGF are very similar to VEGF165. Of all the different isoforms of VEGF, the VEGF165 has the highest bioavailability and biological potency. VEGFA action is mediated by VEGF receptor-1 (VEGFR-1) and VEGFR-2. VEGFR-1 (180 kDa, Flt-1) expression is upregulated by hypoxic conditions in the inner core of tumor mass. VEGFR-2 (200 kDa, KDR) is a major mediator of mitogenic and angiogenic permeability enhancing effects of VEGF. It is also reported to mediate survival signals in endothelial cells.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to mouse VEGF-A near the N-terminus.

Application

Anti-VEGF-A Antibody is an antibody against VEGF-A for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected VEGF-A in mouse kidney, mouse cerebellum, mouse spleen, and rat kidney tissue.

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected VEGF-A in 10 µg of mouse lung tissue lysate.

Target description

~27 kDa observed

Linkage

Replaces: AB1876

Physical form

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_001273985. UniProt accession no.: Q00731. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Vegfa(22339)
rat ... Vegfa(83785). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.