Anti-Vesicular GABA Transporter Antibody (C15-1315-969)

General description

Vesicular GABA transporter (VGAT), the first vesicular amino acid transporter to be molecularly identified, differs in structure from previously cloned vesicular neurotransmitter transporters and defines a novel gene family. VGAT is highly concentrated in the nerve endings of GABAergic neurons in the brain and spinal cord but also in glycinergic nerve endings. In contrast, hippocampal mossy fiber boutons, which are glutamatergic but known to contain GABA, lack VGAT immunoreactivity. Although the great majority of nerve terminals containing GABA or glycine are immunopositive for VGAT, subpopulations of nerve endings rich in GABA or glycine appear to lack the protein. Additional vesicular transporters or alternative modes of release may therefore contribute to the inhibitory neurotransmission mediated by these two amino acids.

Specificity

Cat. # AB2257 recognizes VGAT.

Immunogen

Epitope: C-terminus

KLH conjugated Vesicular GABA Transporter corresponding to the C-terminus.

Application

Anti-Vesicular GABA Transporter Antibody is an antibody against Vesicular GABA Transporter for use in WB, IH.

Immunohistochemistry Analysis:
1:50 - 1:500 dilution from a previous lot detected vesiluar GABA transporter using IHC-Select Detection with HRP-DAB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:500 - 1:1,000 dilution of this lot detected vesicular GABA transporter on 10 µg of rat brain lysate.

Target description

Approx. 57 kDa

Physical form

Antigen Affinity Purified

Rabbit polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Rat brain lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. species reactivity (predicted by homology): monkey, human. technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_080552.2. UniProt accession no.: Q9H598. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SLC32A1(140679)
mouse ... Slc32A1(22348). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.