Anti-Vesicular Glutamate Transporter 1 Antibody (C15-1316-397)

General description

Vesicular glutamate transporter 1 (VGLUT1) is encoded by the gene mapped to human chromosome 19q13. VGLUT1 is specifically expressed in neuronal cells in the brain. It is characterized with a conserved C-terminal dileucine-like motif and two polyproline domains distal to C- terminal end, including one that binds to endocytic BAR (Bin/Amphiphysin/Rvs) domain protein, endophilin.

Specificity

Recognizes Vesicular Glutamate Transporter 1 (VGLUT1, BNPI {Brain specific Na+ dependent inorganic phosphate cotransporter}). The antiserum has been tested on tissue sections from the rat central nervous system (CNS) using immunofluorescence histochemistry. The antiserum mainly labels nerve fibers and terminals. VGLUT1 is an excellent marker for glutamatergic nerve terminals. The staining pattern for the staining obtained with the VGLUT1 antiserum corresponds to the pattern described using other antisera to VGLUT1 (Bellocchio et al., 1998; Fremeau et al., 2001; Fujiyama et al., 2001; Sakata-Haga et al., 2001; Tong et al., 2001; Kaneko et al. 2002; Varoqui et al., 2002). Preabsorption of the VGLUT1 antiserum with immunogen peptide eliminates all immunostaining.

Immunogen

Synthetic peptide from rat VGLUT1 protein with no overlap to VGLUT2. The immunogen is available as AG208.

Application

Anti-Vesicular Glutamate Transporter 1 Antibody has been used in:

• immunohistochemistry(10)
• immunohistochemical analysis
• immunocytochemistry

Biochem/physiol Actions

Vesicular glutamate transporter 1 (VGLUT1) plays a vital role as an vesicular glutamate transporter in glutamatergic neurons. VGLUT1 expression in the prefrontal cortex, functions as a potential neurochemical marker of dementia in Alzheimer disease (AD) and implicated in antidepressant activity.

Target description

62 kDa

Physical form

Guinea pig Serum in 0.1% Sodium Azide.

Other Notes

Concentration: Variable

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: guinea pig. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable. NCBI accession no.: NP_446311. UniProt accession no.: Q62634. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: rat ... Slc17A7(116638). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.