Anti-Vesicular Monoamine Transporter 2 Antibody (C15-1315-847)

Specificity

Recognizes Vesicular Monoamine Transporter 2 (VMAT2). An antibody made to the C-terminal VMAT2 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT2 and not in wild type cells (Peter et al. 1995; Nirenberg et al. 1995). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by the inclusion of protease inhibitors. Higher molecular weight bands may represent glycosylated VMAT2 (Peter et al. 1995; Nirenberg et al. 1995, Liu et al. 1994). VMAT2 has been localized in adrenal chromaffin cells, sympathetic ganglion cells, enterochromaffin-like cells of the stomach and in CNS (Peter et al. 1995; Nirenberg et al. 1995; Nirenberg et al. 1996). May recognize human due to the 92% homology between rat and human VMAT2.

Immunogen

A 13 amino acid C-terminal peptide sequence from rat VMAT2 (Liu et al. 1992). This peptide is predicted to be cytoplasmic.

Application

Anti-Vesicular Monoamine Transporter 2 Antibody is an antibody against Vesicular Monoamine Transporter 2 for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 μg/mL (Chemiluminescence technique)

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 μg/mL (1 μg/mL VMAT1 peptide (Catalog Number AG251)/well)

Optimal working dilutions must be determined by end user.

Target description

55 kDa

Physical form

ImmunoAffinity Purified

Liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_003054.3. UniProt accession no.: Q05940. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... SLC18A2(6571). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.