Anti-VGluT1 (C15-1317-401)

General description

Vesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).

Specificity

This rabbit polyclonal antibody detects Vesicular glutamate transporter 1 in Human, Mouse, and Rat brain. It targets an epitope with in 14 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGLUT1).

Application

Anti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunoautoradiography Analysis: A representative lot detected VGLUT1 in rat and mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

Research Category
Neuroscience

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 20 µg of mouse brain tissue lysate.

Target description

~55 kDa observed; 61.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: unpurified. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human, rat. species reactivity (predicted by homology): bovine (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. L. technique(s): immunoautoradiography: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_446311. UniProt accession no.: Q62634. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... SLC17A7(57030). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.