ANTI-VGLUT1 CLONE 3C10.2 ZOOMAB« MOUSE MONOCLONAL (C005B-262654)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3C10.2 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Vesicular Glutamate Transporter 1 (VGLUT1).

Immunogen

Recombinant rat Vesicular Glutamate Transporter-1 (VGLUT1).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGLUT1 in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected VGLUT1 in Human brain and Mouse brain tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected VGLUT1 in Rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VGLUT1 in SH-SY5Y cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected VGLUT1 in Human brain and Rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Vesicular glutamate transporter 1 (UniProt: Q62634; also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID:  116638) in rat.  VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons.  It mediates glutamate uptake into synaptic vesicles that is driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex, and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Preobraschenski, J., et al. (2018). Cell Rep. 23(2); 535-545; Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18); 7158-7163; Takamori, S., et al. (2000). Nature. 407(6801); 189-194).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.